Review



1x towbin transfer buffer  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 98
      Buy from Supplier

    Structured Review

    Bio-Rad 1x towbin transfer buffer
    1x Towbin Transfer Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 22183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/towbin+1x+transfer+buffer/Tris/pmc06961695-237-28-21
    Average 98 stars, based on 22183 article reviews
    1x towbin transfer buffer - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: LARP1 is a major phosphorylation substrate of mTORC1
    Article Snippet: All proteins were analyzed as aforementioned with exception to 4E-BPs; the latter were resolved on 13.5% (w/v) acrylamide gels (1.5mm thickness) containing 0.36% (w/v) bis N,N’-methylene bisacrylamide (BioRad, catalogue no. 161-0201) at a ratio of 37.5:1 of acrylamide to bis N,N’-methylene bisacrylamide). .. Proteins were then transferred onto a 0.2 μm nitrocellulose membrane (BioRad, catalogue no. 1620112) for 1h at 100V constant by wet (immersion) transfer in a modified Towbin 1x transfer buffer (25 mM Tris, 192 mM glycine at pH 8.3) ( ) containing high (20% (v/v)) methanol and 0.1% (w/v) sodium dodecyl sulfate (SDS). ..

    Article Title: LARP1 is a major phosphorylation substrate of mTORC1
    Article Snippet: Samples of lysates were resolved on 10% (w/v) acrylamide (Millipore Sigma, catalogue no. A3553-500G) gel (1.5mm thickness) containing 0.1% (w/v) bis N,N’-methylene bisacrylamide (BioRad, catalogue no. 161-0201) at a ratio of 100:1 of acrylamide to bis N,N’-methylene bisacrylamide. .. Proteins were then transferred onto a 0.2 μm nitrocellulose membrane (BioRad, catalogue no. 1620112) for 1 h 30 min at 100 V constant by wet (immersion) transfer in a modified Towbin 1x transfer buffer (25 mM Tris, 192 mM glycine at pH 8.3) (77) containing low (10% (v/v)) methanol and 0.1% (w/v) sodium dodecyl sulfate (SDS) for easier transfer of larger molecular weight proteins. .. The membrane was then blocked in 5% (w/v) defatted milk suspended in Tris buffer saline (1xTBS, 150 mM NaCl, 2.7 mM KCl and 24.7 mM Tris base at pH 7.6) containing 0.02% (v/v) Tween20 (TBS-T) for 1 h at room temperature followed by incubation with primary antibodies in 5% bovine serum albumin (BSA heat shock fraction) (Millipore Sigma, catalogue no. A7906) in TBS-T overnight at 4°C on an orbital shaker.

    Article Title: LARP1 is a major phosphorylation substrate of mTORC1
    Article Snippet: SDS Proteins were eluted from the strip by adding isoelectric focusing (IEF) gel sample buffer (supplied with kit) containing 50% (v/v) glycerol (BioRad, catalogue no. 161-0763) and coomassie blue R-250 stain for sample visualization. .. Proteins were resolved by electrophoresis in 1x Tris/glycine/SDS running buffer containing (25 mM Tris, 192 mM glycine, 0.1% (w/v) sodium dodecyl sulfate (SDS) at pH 8.3) at 150 V constant for approximately 1 h 30 min to 2 h. Proteins were then transferred onto a 0.2 μm nitrocellulose membrane (BioRad, catalogue no. 1620112) for 1 h 30 min at 100 V constant by wet (immersion) transfer in a modified Towbin 1x transfer buffer (25 mM Tris, 192 mM glycine at pH 8.3) ( ) containing low (10% (v/v)) methanol and 0.1% (w/v) sodium dodecyl sulfate (SDS) for easier transfer of larger molecular weight proteins. .. Membrane was blocked in 5% (w/v) defatted milk in TBS-T for 1 h at room temperature on an orbital shaker, followed by incubation overnight at 4°C on an orbital shaker with primary anti-human LARP1 antibody (AbCam, catalogue no. 86359) at 1:1000 dilution in 5% (w/v) bovine serum albumin (BSA, heat shock fraction) (Millipore Sigma, catalogue no. A7906) in TBS-T. Unbound primary antibody was washed by incubating membrane twice for 5 min each time in TBS-T.

    Modification:

    Article Title: LARP1 is a major phosphorylation substrate of mTORC1
    Article Snippet: All proteins were analyzed as aforementioned with exception to 4E-BPs; the latter were resolved on 13.5% (w/v) acrylamide gels (1.5mm thickness) containing 0.36% (w/v) bis N,N’-methylene bisacrylamide (BioRad, catalogue no. 161-0201) at a ratio of 37.5:1 of acrylamide to bis N,N’-methylene bisacrylamide). .. Proteins were then transferred onto a 0.2 μm nitrocellulose membrane (BioRad, catalogue no. 1620112) for 1h at 100V constant by wet (immersion) transfer in a modified Towbin 1x transfer buffer (25 mM Tris, 192 mM glycine at pH 8.3) ( ) containing high (20% (v/v)) methanol and 0.1% (w/v) sodium dodecyl sulfate (SDS). ..

    Article Title: LARP1 is a major phosphorylation substrate of mTORC1
    Article Snippet: Samples of lysates were resolved on 10% (w/v) acrylamide (Millipore Sigma, catalogue no. A3553-500G) gel (1.5mm thickness) containing 0.1% (w/v) bis N,N’-methylene bisacrylamide (BioRad, catalogue no. 161-0201) at a ratio of 100:1 of acrylamide to bis N,N’-methylene bisacrylamide. .. Proteins were then transferred onto a 0.2 μm nitrocellulose membrane (BioRad, catalogue no. 1620112) for 1 h 30 min at 100 V constant by wet (immersion) transfer in a modified Towbin 1x transfer buffer (25 mM Tris, 192 mM glycine at pH 8.3) (77) containing low (10% (v/v)) methanol and 0.1% (w/v) sodium dodecyl sulfate (SDS) for easier transfer of larger molecular weight proteins. .. The membrane was then blocked in 5% (w/v) defatted milk suspended in Tris buffer saline (1xTBS, 150 mM NaCl, 2.7 mM KCl and 24.7 mM Tris base at pH 7.6) containing 0.02% (v/v) Tween20 (TBS-T) for 1 h at room temperature followed by incubation with primary antibodies in 5% bovine serum albumin (BSA heat shock fraction) (Millipore Sigma, catalogue no. A7906) in TBS-T overnight at 4°C on an orbital shaker.

    Article Title: LARP1 is a major phosphorylation substrate of mTORC1
    Article Snippet: SDS Proteins were eluted from the strip by adding isoelectric focusing (IEF) gel sample buffer (supplied with kit) containing 50% (v/v) glycerol (BioRad, catalogue no. 161-0763) and coomassie blue R-250 stain for sample visualization. .. Proteins were resolved by electrophoresis in 1x Tris/glycine/SDS running buffer containing (25 mM Tris, 192 mM glycine, 0.1% (w/v) sodium dodecyl sulfate (SDS) at pH 8.3) at 150 V constant for approximately 1 h 30 min to 2 h. Proteins were then transferred onto a 0.2 μm nitrocellulose membrane (BioRad, catalogue no. 1620112) for 1 h 30 min at 100 V constant by wet (immersion) transfer in a modified Towbin 1x transfer buffer (25 mM Tris, 192 mM glycine at pH 8.3) ( ) containing low (10% (v/v)) methanol and 0.1% (w/v) sodium dodecyl sulfate (SDS) for easier transfer of larger molecular weight proteins. .. Membrane was blocked in 5% (w/v) defatted milk in TBS-T for 1 h at room temperature on an orbital shaker, followed by incubation overnight at 4°C on an orbital shaker with primary anti-human LARP1 antibody (AbCam, catalogue no. 86359) at 1:1000 dilution in 5% (w/v) bovine serum albumin (BSA, heat shock fraction) (Millipore Sigma, catalogue no. A7906) in TBS-T. Unbound primary antibody was washed by incubating membrane twice for 5 min each time in TBS-T.

    Molecular Weight:

    Article Title: LARP1 is a major phosphorylation substrate of mTORC1
    Article Snippet: Samples of lysates were resolved on 10% (w/v) acrylamide (Millipore Sigma, catalogue no. A3553-500G) gel (1.5mm thickness) containing 0.1% (w/v) bis N,N’-methylene bisacrylamide (BioRad, catalogue no. 161-0201) at a ratio of 100:1 of acrylamide to bis N,N’-methylene bisacrylamide. .. Proteins were then transferred onto a 0.2 μm nitrocellulose membrane (BioRad, catalogue no. 1620112) for 1 h 30 min at 100 V constant by wet (immersion) transfer in a modified Towbin 1x transfer buffer (25 mM Tris, 192 mM glycine at pH 8.3) (77) containing low (10% (v/v)) methanol and 0.1% (w/v) sodium dodecyl sulfate (SDS) for easier transfer of larger molecular weight proteins. .. The membrane was then blocked in 5% (w/v) defatted milk suspended in Tris buffer saline (1xTBS, 150 mM NaCl, 2.7 mM KCl and 24.7 mM Tris base at pH 7.6) containing 0.02% (v/v) Tween20 (TBS-T) for 1 h at room temperature followed by incubation with primary antibodies in 5% bovine serum albumin (BSA heat shock fraction) (Millipore Sigma, catalogue no. A7906) in TBS-T overnight at 4°C on an orbital shaker.

    Article Title: LARP1 is a major phosphorylation substrate of mTORC1
    Article Snippet: SDS Proteins were eluted from the strip by adding isoelectric focusing (IEF) gel sample buffer (supplied with kit) containing 50% (v/v) glycerol (BioRad, catalogue no. 161-0763) and coomassie blue R-250 stain for sample visualization. .. Proteins were resolved by electrophoresis in 1x Tris/glycine/SDS running buffer containing (25 mM Tris, 192 mM glycine, 0.1% (w/v) sodium dodecyl sulfate (SDS) at pH 8.3) at 150 V constant for approximately 1 h 30 min to 2 h. Proteins were then transferred onto a 0.2 μm nitrocellulose membrane (BioRad, catalogue no. 1620112) for 1 h 30 min at 100 V constant by wet (immersion) transfer in a modified Towbin 1x transfer buffer (25 mM Tris, 192 mM glycine at pH 8.3) ( ) containing low (10% (v/v)) methanol and 0.1% (w/v) sodium dodecyl sulfate (SDS) for easier transfer of larger molecular weight proteins. .. Membrane was blocked in 5% (w/v) defatted milk in TBS-T for 1 h at room temperature on an orbital shaker, followed by incubation overnight at 4°C on an orbital shaker with primary anti-human LARP1 antibody (AbCam, catalogue no. 86359) at 1:1000 dilution in 5% (w/v) bovine serum albumin (BSA, heat shock fraction) (Millipore Sigma, catalogue no. A7906) in TBS-T. Unbound primary antibody was washed by incubating membrane twice for 5 min each time in TBS-T.

    Electrophoresis:

    Article Title: LARP1 is a major phosphorylation substrate of mTORC1
    Article Snippet: SDS Proteins were eluted from the strip by adding isoelectric focusing (IEF) gel sample buffer (supplied with kit) containing 50% (v/v) glycerol (BioRad, catalogue no. 161-0763) and coomassie blue R-250 stain for sample visualization. .. Proteins were resolved by electrophoresis in 1x Tris/glycine/SDS running buffer containing (25 mM Tris, 192 mM glycine, 0.1% (w/v) sodium dodecyl sulfate (SDS) at pH 8.3) at 150 V constant for approximately 1 h 30 min to 2 h. Proteins were then transferred onto a 0.2 μm nitrocellulose membrane (BioRad, catalogue no. 1620112) for 1 h 30 min at 100 V constant by wet (immersion) transfer in a modified Towbin 1x transfer buffer (25 mM Tris, 192 mM glycine at pH 8.3) ( ) containing low (10% (v/v)) methanol and 0.1% (w/v) sodium dodecyl sulfate (SDS) for easier transfer of larger molecular weight proteins. .. Membrane was blocked in 5% (w/v) defatted milk in TBS-T for 1 h at room temperature on an orbital shaker, followed by incubation overnight at 4°C on an orbital shaker with primary anti-human LARP1 antibody (AbCam, catalogue no. 86359) at 1:1000 dilution in 5% (w/v) bovine serum albumin (BSA, heat shock fraction) (Millipore Sigma, catalogue no. A7906) in TBS-T. Unbound primary antibody was washed by incubating membrane twice for 5 min each time in TBS-T.



    Similar Products

    1x towbin transfer buffer  (Bio-Rad)
    98
    Bio-Rad 1x towbin transfer buffer
    1x Towbin Transfer Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/towbin+1x+transfer+buffer/Tris/pmc06961695-237-28-21
    Average 98 stars, based on 1 article reviews
    1x towbin transfer buffer - by Bioz Stars, 2026-09
    98/100 stars
    		  Buy from Supplier
    1x towbin transfer buffer  (Thermo Fisher)
    99
    Thermo Fisher 1x towbin transfer buffer
    1x Towbin Transfer Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/towbin+1x+transfer+buffer/Glycine/pmc06961695-296-28-45
    Average 99 stars, based on 1 article reviews
    1x towbin transfer buffer - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results